Phospho-Histone H3 (Thr3) monoclonal antibody
Product Specifications
Background
H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
CAS Number
9007-83-4
Structure Composition
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Product Name Alternative
H3 histone; family 3A; H3 histone; family 3B (H3.3B) ; H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3
Swiss Prot
P68431
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Thr3 of human Histone H3
Conjugation
Unconjugated
Applications
WB, ICC/IF
Dilution
WB: 1/500-1/1000 IF: 1/50-1/200
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 15 kDa; Observed MW: 15 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-Histone H3 in C6, 3T3, Hela lysates using Phospho-Histone H3 antibody.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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