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Phospho-SIRT1 (Ser47) polyclonal antibody

Product Specifications

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA.

CAS Number

9007-83-4

Structure Composition

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Product Name Alternative

SIRT1; SIR2L1; NAD-dependent protein deacetylase sirtuin-1; hSIRT1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2

Swiss Prot

Q96EB6

Reactivity

Human

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser47 of human SIRT1

Conjugation

Unconjugated

Applications

WB, IHC-F, IHC-P, ICC/IF

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 82 kDa; Observed MW: 120 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-SIRT1 in A549 lysates using Phospho-SIRT1 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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