4E BP1 polyclonal antibody
Product Specifications
Background
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.
CAS Number
9007-83-4
Structure Composition
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Product Name Alternative
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Swiss Prot
Q13541
Reactivity
Human
Immunogen
A synthetic peptide of human eIF4EBP1
Conjugation
Unconjugated
Applications
WB, IHC-P, FC, IP
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20 FC: 1/50-1/100
Purity
Affinity Purified
Modification
Unmodified
Molecular Weight
Calculated MW: 13 kDa; Observed MW: 15-20 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of 4EBP1 in K562, 293, Hela lysates using 4E BP1 antibody.
Host or Source
Rabbit
Available Sizes
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