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Phospho-c-Jun (Ser63) polyclonal antibody

Product Specifications

Background

C-jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Name Alternative

Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39

Swiss Prot

P05412

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-c-Jun (S63)

Conjugation

Unconjugated

Applications

WB, IHC-P, ICC/IF

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 36 kDa; Observed MW: 48 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of c-Jun Phosphorylation in NIH/3T3 lysates treated with Anisomycin using Phospho-c-Jun antibody.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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