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Phospho-BRAF (Thr401) polyclonal antibody

Product Specifications

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.

Structure Composition

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Name Alternative

BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

Swiss Prot

P15056

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-B Raf (T401)

Conjugation

Unconjugated

Applications

WB, ICC/IF, IP

Dilution

WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 84 kDa; Observed MW: 84 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-B Raf in PC-12 lysates; PC-12 lysates treated with TPA using Phospho-BRAF antibody.

Host or Source

Rabbit

CAS Number

9007-83-4

Available Sizes

Curated Selection

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