Phospho-Raf1 (Ser259) polyclonal antibody
Product Specifications
Background
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Product Name Alternative
RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Swiss Prot
P04049
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human Phospho-Raf1 (S259)
Conjugation
Unconjugated
Applications
WB, IHC-P
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 73 kDa; Observed MW: 73 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-Raf1 in 293T lysates; 293T lysates treated with Okadaic Acid + Calyculin A using Phospho-Raf1 antibody.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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