Rb polyclonal antibody
Product Specifications
Background
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
Swiss Prot
P06400
Reactivity
Human, Rat, Mouse
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RB.
Conjugation
Unconjugated
Applications
WB, IHC, ICC/IF, IP
Dilution
WB: 1/500-1/1000 IHC: 1/100-1/200 IF/ICC: 1/100-1/500 IP: 1/10-1/50
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 110 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Rb polyclonal antibody detects endogenous levels of Rb protein.
Applications Notes
Western blot analysis of Rb expression in PC3 (A), Hela (B), NIH3T3 (C) whole cell lysates.
Host or Source
Rabbit
Available Sizes
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