Histone H3.3 polyclonal antibody
Product Specifications
Background
H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Product Name Alternative
H3F3; H3.3A
Swiss Prot
P84243
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human Histone H3.3
Conjugation
Unconjugated
Applications
WB, IHC-P, ICC/IF
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Purity
Affinity Purified
Modification
Unmodified
Molecular Weight
Calculated MW: 15 kDa; Observed MW: 15 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Histone H3.3 in HeLa lysates; NIH/3T3 lysates using Histone H3.3 antibody.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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