Ubiquitin polyclonal antibody
Product Specifications
Background
Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Product Name Alternative
FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A
Swiss Prot
P0CG47
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human Ubiquitin
Conjugation
Unconjugated
Applications
WB, IHC-P, ICC/IF, FC
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100
Purity
Affinity Purified
Modification
Unmodified
Molecular Weight
Calculated MW: 26 kDa; Observed MW: 8 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Ubiquitin in 293T lysates using Ubiquitin antibody.
Host or Source
Rabbit
Available Sizes
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