Phospho-eIF4E (Ser209) polyclonal antibody

Product Specifications

Background

EIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Name Alternative

EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Swiss Prot

P06730

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-eIF4E (S209)

Conjugation

Unconjugated

Applications

WB, IHC-P, ICC/IF, IP

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 25 kDa; Observed MW: 25 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of eIF4E in HEK293 lysates; mouse spleen lysates using Phospho-eIF4E antibody.

Host or Source

Rabbit

Available Sizes

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