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Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) polyclonal antibody

Product Specifications

Background

PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Name Alternative

ADRB2; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; P65-PAK; P68-PAK; PAK1 (phospho S144) ; PAK2 (phospho S141) ; PAK3 (phospho S154)

Swiss Prot

O75914/Q13153/Q13177

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-PAK1/2/3 (S144+S141+S139)

Conjugation

Unconjugated

Applications

WB, IHC-P, ICC/IF, IP, FC

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 61 kDa; Observed MW: 65 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-PAK1/2/3 in HeLa lysates treated with lambda phosphatase using Phospho-PAK1/2/3 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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