Histone H2A.X polyclonal antibody
Product Specifications
Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Product Name Alternative
H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
Swiss Prot
P16104
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human Histone H2A.X
Conjugation
Unconjugated
Applications
WB, IHC-P, ICC/IF, IP
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Purity
Affinity Purified
Modification
Unmodified
Molecular Weight
Calculated MW: 15 kDa; Observed MW: 15 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Histone H2A.X in Raji lysates using Histone H2A.X antibody.
Host or Source
Rabbit
Available Sizes
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