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Histone H2A.X polyclonal antibody

Product Specifications

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Name Alternative

H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X

Swiss Prot

P16104

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Histone H2A.X

Conjugation

Unconjugated

Applications

WB, IHC-P, ICC/IF, IP

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Purity

Affinity Purified

Modification

Unmodified

Molecular Weight

Calculated MW: 15 kDa; Observed MW: 15 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Histone H2A.X in Raji lysates using Histone H2A.X antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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