Phospho-HSF1 (Ser326) polyclonal antibody
Product Specifications
Background
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Product Name Alternative
HSF1; HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1
Swiss Prot
Q00613
Reactivity
Human
Immunogen
A synthesized peptide derived from human Phospho-HSF1 (S326)
Conjugation
Unconjugated
Applications
WB, IHC-P, ICC/IF, IP, FC
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 57 kDa; Observed MW: 82 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-HSF1 in HeLa lysates treated with heat using Phospho-HSF1 antibody.
Host or Source
Rabbit
Available Sizes
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