PHD1 polyclonal antibody
Product Specifications
Background
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Product Name Alternative
Estrogen-induced tag 6; HPH-3; PHD1
Swiss Prot
Q96KS0
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human PHD1/prolyl hydroxylase
Conjugation
Unconjugated
Applications
WB, IHC-P, ICC/IF, FC
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100
Purity
Affinity Purified
Modification
Unmodified
Molecular Weight
Calculated MW: 44 kDa; Observed MW: 44 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of PHD1 in HeLa lysates; A549 lysates using PHD1 antibody.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
LS005071 100 µg
Parkin (PARK2) (NM_013987) Human Over-expression Lysate
Sign In for Pricing
View Details A01631-1-iFluor647 100 µg
Anti-MVD Antibody Picoband® (iFluor647 Conjugate)
Sign In for Pricing
View Details 