Raf1 polyclonal antibody
Product Specifications
Background
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Swiss Prot
P04049
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:251-300
Conjugation
Unconjugated
Applications
ICC/IF, WB, IHC-F, IHC-P, ELISA
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Purity
Affinity Purified
Modification
Unmodified
Molecular Weight
Calculated MW: 73 kDa; Observed MW: 73 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody.
Host or Source
Rabbit
Available Sizes
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