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Phospho-Cardiac Troponin I (Ser22/Ser23) polyclonal antibody

Product Specifications

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM) .

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

TNNI3; TNNC1; Troponin I; cardiac muscle; Cardiac troponin I

Swiss Prot

P23693

Reactivity

Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from rat TNNI3 around the phosphorylation site of Ser22 and Ser23. AA range:5-54

Conjugation

Unconjugated

Applications

WB, IHC-P, ELISA

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 24 kDa; Observed MW: 28 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-Cardiac Troponin I in SH-SY5Y lysates using Phospho-Cardiac Troponin I antibody.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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