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Phospho-JAK2 (Tyr1007) polyclonal antibody

Product Specifications

Background

Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2

Swiss Prot

O60674

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr1007. AA range:981-1030

Conjugation

Unconjugated

Applications

WB, IHC-P, ELISA

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 131 kDa; Observed MW: 131 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-JAK2 in NIH3T3 lysates using Phospho-JAK2 antibody.

Host or Source

Rabbit

CAS Number

9007-83-4

Available Sizes

Curated Selection

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