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Phospho-IRS1 (Ser639) polyclonal antibody

Product Specifications

Background

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit .

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

IRS1; Insulin receptor substrate 1; IRS-1

Swiss Prot

P35568

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser639. AA range:605-654

Conjugation

Unconjugated

Applications

WB, IHC-P, ELISA

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 132 kDa; Observed MW: 150 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-IRS1 in various lysates using Phospho-IRS1 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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