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Cleaved-Caspase 3 p17 polyclonal antibody

Product Specifications

Background

Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Swiss Prot

P42574

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:126-175

Conjugation

Unconjugated

Applications

WB, ICC/IF, IHC-F, IHC-P, ELISA

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Purity

Affinity Purified

Modification

Cleaved

Molecular Weight

Calculated MW: 32 kDa; Observed MW: 17 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Cleaved-Caspase3 in various lysates using Cleaved-Caspase3 p17 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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