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Phospho-NF-KB p65 (Ser536) polyclonal antibody

Product Specifications

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1) /p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB

Swiss Prot

Q04206

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser536. AA range:502-551

Conjugation

Unconjugated

Applications

ICC/IF, WB, IHC-F, IHC-P, IP, ELISA

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ELISA: 1/10000

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 60 kDa; Observed MW: 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-NF-KB p65 in various lysates using Phospho-NF-KB p65 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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