Phospho-Rb (Ser780) polyclonal antibody

Product Specifications

Background

Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

Swiss Prot

P06400

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800

Conjugation

Unconjugated

Applications

WB, IHC-F, IHC-P, ICC/IF, ELISA

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 106 kDa; Observed MW: 106 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-Rb in various lysates using Phospho-Rb antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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