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Phospho-IKB alpha (Ser32/Ser36) polyclonal antibody

Product Specifications

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3

Swiss Prot

P25963

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Ser32/Ser36. AA range:15-64

Conjugation

Unconjugated

Applications

WB, IHC-F, IHC-P, ICC/IF, ELISA

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 36 kDa; Observed MW: 36 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-IKB alpha in various lysates using Phospho-IKB alpha antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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