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Phospho-Chk2 (Thr68) polyclonal antibody

Product Specifications

Background

These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2

Swiss Prot

O96017

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68. AA range:35-84

Conjugation

Unconjugated

Applications

WB, ICC/IF, IHC-P

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 61 kDa; Observed MW: 61 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-Chk2 in Jurkat treated with UV lysates using Phospho- Chk2 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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