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Phospho-AMPK alpha 1/2 (Thr183/Thr172) polyclonal antibody

Product Specifications

Background

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3) .

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

PRKAA1; AMPK1; 5' -AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK

Swiss Prot

Q13131/P54646

Reactivity

Human, Mouse, Rat, Monkey, Pig

Immunogen

The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Conjugation

Unconjugated

Applications

ICC/IF, WB, IHC-F, IHC-P, ELISA

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 64 kDa; Observed MW: 64 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-AMPK alpha 1/2 in various lysates using Phospho-AMPK alpha 1/2 antibody.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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