HIF1 alpha polyclonal antibody
Product Specifications
Background
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT) . This gene encodes the alpha subunit of HIF-1.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma
Swiss Prot
Q16665
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377
Conjugation
Unconjugated
Applications
ICC/IF, WB, IHC-F, IHC-P, ELISA
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Purity
Affinity Purified
Modification
Unmodified
Molecular Weight
Calculated MW: 93 kDa; Observed MW: 92-130 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of HIF1 alpha in LOVO lysates using HIF1 alpha antibody.
Host or Source
Rabbit
Available Sizes
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