Phospho-Histone H3 (Ser10) polyclonal antibody
Product Specifications
Background
H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
H3 histone; family 3A; H3 histone; family 3B (H3.3B) ; H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3
Swiss Prot
P68431
Reactivity
Human
Immunogen
Human Phospho-Histone H3 (Ser10) synthetic peptide conjugated to KLH.
Conjugation
Unconjugated
Applications
WB, ICC/IF
Dilution
WB: 1/500-1/1000 IF: 1/50-1/200
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 15 kDa; Observed MW: 15 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-Histone H3 in HeLa lysates, untreated or 1mM hydroxyurea treated for 5 hours, using Phospho-Histone H3 antibody.
Host or Source
Rabbit
Available Sizes
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