Phospho-LAT (Tyr191) polyclonal antibody
Product Specifications
Background
Required for TCR (T-cell antigen receptor) - and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III) -mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor) -mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
LAT; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38
Swiss Prot
O43561
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human LAT around the phosphorylation site of Tyr191. AA range:191-240
Conjugation
Unconjugated
Applications
WB, IHC-P, ELISA
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 28 kDa; Observed MW: 28 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Phospho-LAT antibody.High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.Sample with blocking peptide on the right.
Host or Source
Rabbit
Available Sizes
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