Phospho-FOXO1/3 (Ser322/S325) polyclonal antibody
Product Specifications
Background
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1
Swiss Prot
Q12778/O43524
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human FOXO1A/3A around the phosphorylation site of Ser322 and Ser325. AA range:291-340
Conjugation
Unconjugated
Applications
WB, IHC-P, ELISA
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 70 kDa; Observed MW: 97 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-FOXO1/3 in 3T3 lysates using Phospho-FOXO1/3 antibody.
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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