PI3 Kinase p85 alpha (3H5) monoclonal antibody

Product Specifications

Background

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha

Swiss Prot

P27986

Reactivity

Human, Mouse, Rat

Immunogen

Purified recombinant protein expressed in E.coli.

Conjugation

Unconjugated

Applications

WB, IHC-P

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100

Purity

Affinity Purified

Modification

Unmodified

Molecular Weight

Calculated MW: 84 kDa; Observed MW: 84 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG1

Applications Notes

Immunohistochemistry analysis of paraffin-embedded rat Liver Tissue using PI3 Kinase p85 alpha antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.