ATG7 (1F8) monoclonal antibody

Product Specifications

Background

The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

HAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L

Swiss Prot

O95352

Reactivity

Human, Rat, Mouse

Immunogen

Purified recombinant protein expressed in E.coli.

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC: 1/50-1/100

Purity

Affinity Purified

Modification

Unmodified

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG1

Applications Notes

Immunohistochemistry analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG7 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.