Acetyl-p53 (Lys382) (3E3) monoclonal antibody

Product Specifications

Background

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

Cellular tumor antigen p53; Cys 51 stop; Tp53; Tumor protein p53

Swiss Prot

P04637

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide conjugated to KLH.

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC: 1/50-1/100

Purity

Affinity Purified

Modification

Acetylated

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG1

Applications Notes

Immunohistochemistry analysis of paraffin-embedded Human Colon Carcinoma Tissue using Acetyl-p53 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.