Smad2/3 (6F7) monoclonal antibody
Product Specifications
Background
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 . Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses .
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Swiss Prot
P84022/Q15796
Reactivity
Human, Rat, Mouse
Immunogen
Recombinant Protein of Smad3
Conjugation
Unconjugated
Applications
IHC-P, WB
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100
Purity
Affinity Purified
Modification
Unmodified
Molecular Weight
Calculated MW: 52 kDa; Observed MW: 52,60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Immunohistochemistry analysis of paraffin-embedded Human Live rTissue using Smad2/3 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Host or Source
Mouse
CAS Number
9007-83-4
Available Sizes
Curated Selection
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