XRCC4 (7C10) monoclonal antibody

Product Specifications

Background

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. Binds to DNA and to DNA ligase IV (LIG4) . The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

X ray repair cross complementing protein 4; DNA repair protein XRCC4; DNA double strand break repair

Swiss Prot

Q13426

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic Peptide of XRCC4

Conjugation

Unconjugated

Applications

WB, IHC-F, IHC-P, ICC/IF, IP

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Purity

Affinity Purified

Modification

Unmodified

Molecular Weight

Calculated MW: 38 kDa; Observed MW: 38-45 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG1

Applications Notes

Immunohistochemistry analysis of paraffin-embedded XRCC4 in Human lung cancer using XRCC4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.