SPARC polyclonal antibody
Product Specifications
Background
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
SPARC; Basement-membrane protein 40; BM-40; Osteonectin; Short name=ON; Secreted protein acidic and rich in cysteine; SPARC; ON
Swiss Prot
P09486
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1) .
Conjugation
Unconjugated
Related Pathways
Contribution of ECM and Cytoskeletal Factors to EMT
Applications
WB IHC-P IF/ICC
Dilution
WB 1:500 - 1:2000 IHC-P 1:50 - 1:100 IF /ICC 1:50 - 1:100
Purity
Modification
Unmodified
Molecular Weight
~ 35 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
SPARC polyclonal antibody detects endogenous levels of SPARC protein.
Applications Notes
Western blot analysis of extracts of A375 cells, using SPARC antibody at 1:3000 dilution.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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