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SPARC polyclonal antibody

Product Specifications

Background

SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

SPARC; Basement-membrane protein 40; BM-40; Osteonectin; Short name=ON; Secreted protein acidic and rich in cysteine; SPARC; ON

Swiss Prot

P09486

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1) .

Conjugation

Unconjugated

Related Pathways

Contribution of ECM and Cytoskeletal Factors to EMT

Applications

WB IHC-P IF/ICC

Dilution

WB 1:500 - 1:2000 IHC-P 1:50 - 1:100 IF /ICC 1:50 - 1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 35 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SPARC polyclonal antibody detects endogenous levels of SPARC protein.

Applications Notes

Western blot analysis of extracts of A375 cells, using SPARC antibody at 1:3000 dilution.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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