TLR4 (S441) polyclonal antibody
Product Specifications
Background
Toll like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti fungal response, while the related 18 wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram negative bacteria.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Toll-like receptor 4; hToll; CD284; TLR-4
Swiss Prot
O00206
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 421-470 of Human TLR4.
Conjugation
Unconjugated
Applications
WB IHC IF IP
Dilution
WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 120 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
TLR4 (S441) polyclonal antibody detects endogenous levels of TLR4 protein.
Applications Notes
Western blot (WB) analysis of TLR4 (S441) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:Myla2059 whole cell lysate (40ug) Lane4:Jurkat whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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