CD161 polyclonal antibody

Product Specifications

Background

Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha (1,3) Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Killer cell lectin-like receptor subfamily B member 1; C-type lectin domain family 5 member B; HNKR-P1a; NKR-P1A; Natural killer cell surface protein P1A; CD161

Swiss Prot

Q12918

Reactivity

Rat, Mouse, Human

Immunogen

Recombinant protein of human CD161.

Conjugation

Unconjugated

Applications

WB IF

Dilution

WB: 1:5000~1:10000 IF 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 43 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD161 polyclonal antibody detects endogenous levels of CD161 protein.

Applications Notes

Western blot (WB) analysis of CD161 polyclonal antibody at 1:5000 dilution Lane1:HEK293T cell membrane lysate (30ug) Lane2:MCF-7 cell membrane lysate (30ug) Lane3:CT-26 whole cell lysate (30ug) Lane4:PC12 whole cell lysate (30ug)