CD48 polyclonal antibody

Product Specifications

Background

CD48 is a glycosylphosphatidylinositol (GPI) -anchored membrane protein of the signaling lymphocyte activation molecule (SLAM) family, also known as SLAMF2 and BLAST-1. It is constitutively expressed on most hematopoietic cells (not on neutrophils and a subset of long-term hematopoietic stem cells in mice) and can be upregulated under certain conditions like infection. Interaction with its low affinity ligand CD2 promotes adhesion and TCR signaling. Interaction with the high affinity ligand CD244 (2B4) regulates natural killer (NK) and CD8 T cell activation and cytolytic function.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

CD48 antigen; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; SLAM family member 2; SLAMF2; Signaling lymphocytic activation molecule 2; TCT.1; CD_antigen: CD48; CD48; BCM1; BLAST1

Swiss Prot

P09326

Reactivity

Human, Rat

Immunogen

Recombinant protein of human CD48.

Conjugation

Unconjugated

Applications

WB IF

Dilution

WB: 1:1000~1:2000 IF 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 48 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD48 polyclonal antibody detects endogenous levels of CD48 protein.

Applications Notes

Western blot (WB) analysis of CD48 polyclonal antibody at 1:1000 dilution Lane1:LOVO whole cell lysate (30ug) Lane2:L02 cell membrane lysate (30ug) Lane3:MCF-7 whole cell lysate (30ug) Lane4:PMVEC whole cell lysate (20ug)