MDFI (P134) polyclonal antibody
Product Specifications
Background
MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. MDFI an also interfere with the DNA-binding activity of Myod family members and plays an important role in trophoblast and chondrogenic differentiation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
MyoD family inhibitor; Myogenic repressor I-mf; MDFI
Swiss Prot
Q99750
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 100-146 of Human MDFI.
Conjugation
Unconjugated
Applications
WB IF
Dilution
WB: 1:500~1:1000 IF 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 25 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MDFI (P134) polyclonal antibody detects endogenous levels of MDFI protein.
Applications Notes
Western blot (WB) analysis of MDFI (P134) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:C6 whole cell lysate (40ug) Lane6:3T3-L1 whole cell lysate (40ug)
Host or Source
Rabbit
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