C/EBP-α (M15) polyclonal antibody
Product Specifications
Background
C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA-binding activity of C/EBP α. C/EBP α functions as an important transcription factor that regulates different genes, including Prolactin gene expression.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; C/EBP α; C/EBPα
Swiss Prot
P49715
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids N-terminus of Human C/EBP-α.
Conjugation
Unconjugated
Related Pathways
ESC Pluripotency and Differentiation Signaling Pathway
Applications
WB IHC
Dilution
WB 1:500 - 1:1000 IHC 1:50 - 1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 43 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
C/EBP-α (M15) polyclonal antibody detects endogenous levels of C/EBP-α protein.
Applications Notes
Western blot analysis of C/EBP alpha expression in mouse embryo (A), rat ovary (B), NIH3T3L1 (C), PC12 (D), Hela (E), Panc1 (F), SGC7901 (G) whole cell lysates.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
