Caspase-3 (Phospho-S150) polyclonal antibody

Product Specifications

Background

Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) . Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12; CASP3; CPP32

Swiss Prot

P42574

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human Caspase-3 around the phosphorylation site of Serine 150.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, Death Receptor Signaling

Applications

Dilution

WB: 1:1000~1:2000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 33 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Caspase-3 (Phospho-S150) polyclonal antibody detects endogenous levels of Caspase-3 protein only when phosphorylated at Ser150.

Applications Notes

Western blot (WB) analysis of Caspase-3 (Phospho-S150) polyclonal antibody at 1:1000 dilution Lane1:Jurkat whole cell lysate (30ug) Lane2:Jurkat treated with λ-phosphatase whole cell lysate (30ug)