Caspase-3 (Phospho-S150) polyclonal antibody
Product Specifications
Background
Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) . Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12; CASP3; CPP32
Swiss Prot
P42574
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human Caspase-3 around the phosphorylation site of Serine 150.
Conjugation
Unconjugated
Related Pathways
Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, Death Receptor Signaling
Applications
WB
Dilution
WB: 1:1000~1:2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 33 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Caspase-3 (Phospho-S150) polyclonal antibody detects endogenous levels of Caspase-3 protein only when phosphorylated at Ser150.
Applications Notes
Western blot (WB) analysis of Caspase-3 (Phospho-S150) polyclonal antibody at 1:1000 dilution Lane1:Jurkat whole cell lysate (30ug) Lane2:Jurkat treated with λ-phosphatase whole cell lysate (30ug)
Host or Source
Rabbit
Available Sizes
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