Frizzled-10 (FZD10) polyclonal antibody

Product Specifications

Background

Receptor for Wnt proteins. Functions in the canonical Wnt/beta-catenin signaling pathway (By similarity) . The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable) .

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Frizzled-10; Fz-10; hFz10; FzE7; CD350; FZD10

Swiss Prot

Q9ULW2

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human Frizzled-10.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Frizzled-10 (FZD10) polyclonal antibody detects endogenous levels of Frizzled-10 protein.

Applications Notes

Western blot (WB) analysis of Frizzled-10 (FZD10) polyclonal antibody at 1:500 dilution Lane1:EC9706 whole cell lysate (15ug) Lane2:Hela whole cell lysate (30ug) Lane3:Myla2059 whole cell lysate (15ug) Lane4:The Brain tissue lysate of Mouse (30ug) Lane5:The Brain tissue lysate of Rat (30ug) Lane6:BV2 whole cell lysate (30ug) Lane7:PMVEC whole cell lysate (30ug)