Tubulin α (9H4) monoclonal antibody

Tubulin is a major cytoskeleton component that has three distinct forms, designated α, β and γ Tubulin. α and β Tubulins form heterodimers, which multimerize to form a microtubule filament. γ Tubulin forms a soluble multiprotein particle with several other proteins. This particle, designated the gammasome, is required for nucleating microtubule filaments at the centrosome. In several organisms, numerous isoforms of the Tubulins exist that are encoded by different genes. The α and β isoforms undergo a variety of post-translational modifications, which may affect microtubule stability and protein interactions. High expression of class II β Tubulin has been seen in elongating axons, indicating a role in neurite outgrowth. Tubulins may also play a role in non-neuronal cell process formation

Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1A; TUBA3; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of α-tubulin (9H4) mAb at 1:5000/10000/20000 dilution Lane1:The Kidney tissue lysate of Mouse (20ug) Lane2:The Kidney tissue lysate of Rat (20ug) Lane3:PC12 whole cell lysate (20ug) Lane4:CT-26 whole cell lysate (20ug) Lane5:HEK293T whole cell lysate (20ug)