Tubulin α (G436) polyclonal antibody

Product Specifications

Background

Tubulin is a major cytoskeleton component that has three distinct forms, designated α, β and γ Tubulin. α and β Tubulins form heterodimers, which multimerize to form a microtubule filament. γ Tubulin forms a soluble multiprotein particle with several other proteins. This particle, designated the gammasome, is required for nucleating microtubule filaments at the centrosome. In several organisms, numerous isoforms of the Tubulins exist that are encoded by different genes. The α and β isoforms undergo a variety of post-translational modifications, which may affect microtubule stability and protein interactions. High expression of class II β Tubulin has been seen in elongating axons, indicating a role in neurite outgrowth. Tubulins may also play a role in non-neuronal cell process formation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1A; TUBA3; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B

Swiss Prot

Q71U36/P68363

Reactivity

Human, Mouse, Rat, Pig, Chicken, Odontobutis obscurus

Immunogen

Synthetic peptide, corresponding to amino acids 201-256 of Human Tubulin α.

Conjugation

Unconjugated

Related Pathways

Regulation of Microtubule Dynamics

Applications

WB IHC IF

Dilution

WB: 1:5000~20000 IHC: 1:100~500 IF: 1:500~1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Tubulin α (G436) polyclonal antibody detects endogenous levels of Tubulin α protein.

Applications Notes

Western blot (WB) analysis of Tubulin α (G436) pAb at 1:5000/1:10000/1:20000 dilution Lane1:A549 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:CT-26 whole cell lysate (40ug)