PI3K p85α (Q498) polyclonal antibody

Product Specifications

Background

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha) . p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R1; GRB1

Swiss Prot

P27986

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 470-510 of Human PI3K p85α.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, PI3K AKT signaling, Protein Kinase C Signaling, Regulation of Actin Dynamics, Warburg Effect, Regulation of Microtubule Dynamics, SAPK JNK Signaling Cascades, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis, MTOR signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 85 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PI 3-kinase p85α (Q498) polyclonal antibody detects endogenous levels of PI 3-kinase p85α protein.

Applications Notes

Western blot (WB) analysis of PI3K p85α (Q498) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:The Brain tissue lysate of Rat (40ug) Lane5:The Lung tissue lysate of Mouse (40ug)