β-Actin (4D3) monoclonal antibody
Product Specifications
Background
Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB; Actin-β; Beta actin
Reactivity
Human, Mouse, Rat, zebrafish, Pig, Chicken, Odontobutis obscurus
Immunogen
Recombinant full length Human β-Actin.
Conjugation
Unconjugated
Related Pathways
Contribution of ECM and Cytoskeletal Factors to EMT, Adherens Junction signaling
Applications
WB IHC IF IP
Dilution
WB: 1:5000~1:20000 IHC: 1:100~1:1000 IF: 1:100~1:500 IP: 1:100~500
Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 42 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
β-Actin (4D3) detects endogenous levels of β-actin protein.
Applications Notes
Western blot (WB) analysis of β-Actin (4D3) mAb at 1:5000-1:20000 dilution Lane1:The Lung tissue lysate of Mouse (20ug) Lane2:The Lung tissue lysate of Rat (20ug) Lane3:H9C2 whole cell lysate (20ug) Lane4:CT26 whole cell lysate (20ug) Lane5:L02 whole cell lysate (20ug) Lane6:HEK293T whole cell lysate (20ug)
Host or Source
Mouse
Available Sizes
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