β-Actin (4D3) monoclonal antibody

Product Specifications

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB; Actin-β; Beta actin

Reactivity

Human, Mouse, Rat, zebrafish, Pig, Chicken, Odontobutis obscurus

Immunogen

Recombinant full length Human β-Actin.

Conjugation

Unconjugated

Related Pathways

Contribution of ECM and Cytoskeletal Factors to EMT, Adherens Junction signaling

Applications

WB IHC IF IP

Dilution

WB: 1:5000~1:20000 IHC: 1:100~1:1000 IF: 1:100~1:500 IP: 1:100~500

Purity

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 42 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

β-Actin (4D3) detects endogenous levels of β-actin protein.

Applications Notes

Western blot (WB) analysis of β-Actin (4D3) mAb at 1:5000-1:20000 dilution Lane1:The Lung tissue lysate of Mouse (20ug) Lane2:The Lung tissue lysate of Rat (20ug) Lane3:H9C2 whole cell lysate (20ug) Lane4:CT26 whole cell lysate (20ug) Lane5:L02 whole cell lysate (20ug) Lane6:HEK293T whole cell lysate (20ug)