Tubulin β polyclonal antibody
Product Specifications
Background
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB3; TUBB4
Reactivity
Human, Mouse, Rat, Odontobutis obscurus
Immunogen
Recombinant full length Human Tubulin β.
Conjugation
Unconjugated
Applications
WB IP IF
Dilution
WB:1:5000~1:20000 IP: 1:100~500 IF: 1:500~1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 55 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Tubulin β polyclonal antibody pAb detects endogenous levels of Tubulin β protein.
Applications Notes
Western blot (WB) analysis of Tubulin β pAb at 1:5000/1:10000/1:20000/1:40000 dilution Lane1:L02 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:MG63 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug) Lane6:The Brain tissue lysate of Rat (40ug) Lane7:The Brain tissue lysate of Mouse (40ug)
Host or Source
Rabbit
Available Sizes
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