Tubulin β polyclonal antibody

Product Specifications

Background

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB3; TUBB4

Reactivity

Human, Mouse, Rat, Odontobutis obscurus

Immunogen

Recombinant full length Human Tubulin β.

Conjugation

Unconjugated

Applications

WB IP IF

Dilution

WB:1:5000~1:20000 IP: 1:100~500 IF: 1:500~1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Tubulin β polyclonal antibody pAb detects endogenous levels of Tubulin β protein.

Applications Notes

Western blot (WB) analysis of Tubulin β pAb at 1:5000/1:10000/1:20000/1:40000 dilution Lane1:L02 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:MG63 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug) Lane6:The Brain tissue lysate of Rat (40ug) Lane7:The Brain tissue lysate of Mouse (40ug)